Absorbance Measurements with the Ossila Spectrofluorometer

Absorbance spectroscopy is capable of revealing critical information about a sample's molecular structure, concentration, and optoelectronic behaviour, and can even track dynamic changes such as shifts in phase or chemical composition. This guide demonstrates how to use the Ossila Spectrophotometer or Spectrofluorometer to take reliable absorbance measurements. 

Absorbance Measurement: Step-by-Step Guide

---

• Open the Ossila Spectrophotometry software on the Absorption tab.  Define the start and end wavelength to determine wavelength range, and wavelength step size (down to 1 nm).
• You initially need to take a reference measurement. This can be with no sample in place, with a blank substrate or with a cuvette filled with solvent.
• To take the measurement, make sure the "Measuring Reference" message is showing under Reference Spectrum. Then press the play button to start the measurement. 
• The reference measurement will appear as a dotted plot showing the transmitted intensity. You should ensure that the parameters for the reference measurement are the same as will be used for your experiment.
• Adjust slit widths and light power to maximize reference spectrum without flooding the detector.  
  
• Once happy with the reference, select it as under the "Reference Spectrum" tab.
• Before taking absorbance measurement, you can enter the name of your sample.
• Place your sample in the dedicated sample holder and start the absorbance measurement.
• Measured data appears as a solid line, with absorbance axis on the left.
• Multiple spectra can be plotted on screen simultaneously. Toggle spectra on and off using the Sample Display Window on the right.
• This window can also be used to view data directly under the data tab.

Spectrofluorometer

Request a quote or buy online

More Resources

---