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Ossila Spectrofluorometer Comparison

Fluorescence Spectrometers

Key Features | Comparison With Competitor | Limitations


The Ossila Spectrofluorometer has everything you need for reliable and sensitive optical spectroscopy measurements. This includes:

  • Two LED light sources (one hyperspectral 380 nm – 1000 nm white LED, one high-power 365 nm UV LED)
  • 2 silicon photomultiplier detectors
  • 2 monochromators
  • A sample chamber with integrated filter holders

Key Features of the Ossila Spectrofluorometer 


Spectrofluorometer configuration used for absorbance measurements
Setup for absorbance measurements in the spectrofluorometer

Low Price

The use of LED light sources, along with clever design and product optimization, means the spectrofluorometer has a great price.

As well as being less expensive than other models, the LED light source has a longer lifetime than most traditional light systems. This also means lowers maintenance costs and less frequent replacement.

Optimized Design

The Ossila Spectrofluorometer has:

  • Optimized alignment of components for both transmission and fluorescence measurements, with the same sample chamber.
  • All reflective optics (no lenses) to eliminate spherical aberrations.
  • Uses silicon photomultiplier detectors with higher sensitivity and a wider range than standard photodiodes. Additionally, these detectors do not require high voltages like other spectrofluorometer set-ups, minimizing lab requirements.
  • Small lab footprint for easy integration into busy labs.
  • Components like LED light sources and detectors are easy to replace, extending the lifetime of the instrument.

Easy-to-Use Software

  • Free plug-and-play software with absorbance, transmission, excitation and emission fluorescence measurements pre-programmed.
  • Part control that makes issue diagnosis and problem solving easy.
  • The instrument can also be controlled via serial commands for specific measurements.
Fluorescence measurements with the Ossila Spectrophotometry Software

LED Light Sources

The LED light sources have a longer operational lifetime then arc lamp alternatives. This also means they require less maintenance, are less dangerous to replace and have to be replaced less often.

LED intensity can be easily altered using the compatible software, with additional light manipulation using the adjustable slits and pre-positioned filters.

For fluorescence measurements, choose between UV light source (at 365 nm) or select a specific wavelength using the tunable light source. This design gives you more flexibility in your experiments.

How Does the Ossila Spectrofluorometer Compare?


We’ve compared our specifications with one of the leading competitors to demonstrate how the Ossila Spectrofluorometers compares.

Leading Competitor Ossila Spectrofluorometer
Accuracy 0.5 nm <1 nm
Repeatability 0.1 nm 1 nm
Reference diode (for measuring light source directly) Yes No
Detector PMT (185-850 nm), High voltage (needs high voltage source) PMT (380-1000 nm), Silicon photomultiplier
Gratings 1200 groove/mm blazed at 330 nm (excitation) and 500 nm (emission), plane ruled 2 x Ruled Reflective Diffraction Grating, 1200/mm, 500 nm Blaze
Monochromators All reflective optics, Czerny-Turner spectrometers All reflective optics, Czerny-Turner configuration
Lamp/ Light sources Vertically mounted, CW, 150 W Ozone-free xenon arc lamp Broadband LED light source (385-1000 nm), UV LED light source (365 nm
Compatible Software Yes Yes
Dimensions 83 cm x 28 cm x 48 cm 28 cm x 26 cm x 10 cm
Price £20000- £45000 £11000

Ossila Spectrofluorometer Measurement Comparison

The Ossila Spectrofluorometer provides a cost-effective alternative for conducting most optical spectroscopy measurements. It is capable of all the basic spectroscopy measurements offered by leading competitors. However, it is primarily for relative, steady-state measurements, where other competitors offer additional measurements.

Leading Competitor Ossila Spectrofluorometer
PL / Fluorescence Yes Yes
PLE Yes Yes
Absorbance Yes Yes
Transmission Yes Yes
Additional Measurements (Acheivable with appropriate additions)
Time Dependent PL Yes Check Mark
PLQY Yes Check Mark

Limitations


  • Lower step size than other spectrophotometers
    • In theory, this could limit resolving power
    • Ossila Spectrofluorometer has suitable resolving power for most optcal features (> 1 nm FWHM)
    • Any measurable absorbance/emission feature below 1 nm FWHM may be limited by spectrophotometer resolving power.
  • Limited in the UV spectrum
    • Same grating used for excitation and emission monochromators
    • Wavelength range 380 – 1000 nm, perfect for measurements within the visible region.
    • Not suitable for measurement or excitation <385 nm and <365 nm respectively.
  • No direct correlating reference measurement
    • One optical pathway
    • No reference pathway or reference diode
    • Reference measurements must be measured separately before absorbance or transmission measurements.
    • Still suitable for most relative spectroscopy measurements.
  • Less broadband light source
    • Light source made of 12 individual LED light sources
    • Still provides broadband excitation 380-1000 nm, while enabling lower costs, longer lifetimes and safer replacement.

Spectrofluorometer

Spectrofluorometer