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Product Code B2451-10g
Price $82 ex. VAT

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A fluorinated nitrobenzoic acid building block

Having multiple functional groups for synthesizing fluorescent sensing molecules, heterocyclic APIs and peptides


2-Fluoro-5-nitrobenzoic acid (CAS number 7304-32-7) is a benzoic acid derivative with substituents of a fluorine and a nitro-group at 2- and 5-positions. Fluorescent probes for detecting nucleophiles such as endogenous hydrogen polysulfide and hydrazine can be synthesised from 2-fluoro-5-nitrobenzoic acid and fluorescent dyes through esterification. The fluorescent probes have intrinsically weak fluorescence, and yet to be turned on by the targeting nucleophiles. The nucleophiles hydrolyse the fluorescent probes (ester hydrolysis), which releases the strong fluorescent dyes observed by fluorescent microscopy.

The fluorine atom substitutes beside the carboxylic group in 2-fluoro-5-nitrobenzoic acid, enabling it to synthesize polycyclic heterocycles and β-turn cyclic peptidomimetics.

Multiple functional groups

Multiple functional groups

For facile synthesis

Fluorinated building block

Fluorinated nitrobenzoic acid building block

For drug discovery, bioimaging and peptide research

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High purity 7304-32-7

High purity

>98% High purity

General Information

CAS Number 7304-32-7
Chemical Formula C7H4FNO4
Full Name 2-Fluoro-5-nitrobenzoic acid
Molecular Weight 185.11 g/mol
Synonyms 2-Fluoro-5-nitrobenzenecarboxylate
Classification / Family Fluorinated building block, fluorescent probes, Heterocyclics, APIs, Peptides

Chemical Structure

2-Fluoro-5-nitrobenzoic acid chemical structure, CAS 7304-32-7.
2-Fluoro-5-nitrobenzoic acid chemical structure, CAS 7304-32-7

Product Details

Purity 98%
Melting Point Tm = 142 °C – 144 °C
Appearance White powder

MSDS Documentation

2-Fluoro-5-nitrobenzoic acid2-Fluoro-5-nitrobenzoic acid MSDS Sheet

Literature and Reviews

  1. Efficient solid-phase synthesis of 2,1,3-benzothiadiazin-4-one 2-oxides with synphaseTM lanterns, S. Makino et al., Bull. Korean Chem. Soc., 24(3), 389–392(2003); DOI: 10.5012/bkcs.2003.24.3.389.
  2. One-pot high-throughput synthesis of β-turn cyclic peptidomimetics via "volatilizable" supports, Y. Li et al., J. Org. Chem., 74(5), 2183–2185(2009); DOI:10.1021/jo802583t.
  3. Synthesis and the crystal structure of (E)-2-(7-(3-(thiophen-2-yl)acrylamido)-2,3-dihydro-5-oxobenzo[e][1,4]oxazepin-1(5H)-yl) ethyl acetate, Y Lee et al., J. Chem. Crystallogr., 39, 902–907(2009); DOI: 10.1007/s10870-009-9588-y.
  4. A Nile red-based near-infrared fluorescent probe for endogenous hydrogen polysulfides in living cells, K.-B. Li et al., Anal. Methods, 9, 6443–6447(2017); DOI: 10.1039/C7AY02376F.
  5. Rapid detection of hydrazine in almost wholly water solution and in living cells with a new colorimetric and fluorescent turn-on probe, Q. Zhai et al., Anal. Methods, 8, 5832–5837(2016); DOI: 10.1039/C6AY01367H.

To the best of our knowledge the information provided here is accurate. The values provided are typical at the time of manufacture and may vary over time and from batch to batch. Products may have minor cosmetic differences (e.g. to the branding) compared to the photos on our website. All products are for laboratory and research and development use only.

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