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Glove Box Installation

The Ossila Glove Box (or glovebox) is designed to be easy to install and maintain, and is suitable for most laboratories. Its small footprint and quick set up also means that it is relatively portable and can be conveniently transferred between labs as required.

To set up the Ossila Glove Box:

  1. Install the moisture and oxygen sensor boards
  2. Secure the gloves on the glove ports
  3. Assemble the window, taking care to ensure a good seal
  4. Seal the feed through ports as required
  5. Choose and fasten the inlet and outlet pipes
  6. Assemble the pressure relief valve
  7. Define settings for the glove box including O2 and H2O levels
The Ossila Glove Box installed in a lab
Initial requirements

The Ossila Glove Box can be easily installed in most laboratories due to its small bench footprint and easy-use software. Successful installation requires:

  • Access to a nitrogen or argon source regulated to at least 2 bar
  • Access to a fume hood or ventilated area to vent outlet gas
  • Access to a plug socket
  • 12mm piping for inlet and outlet tubes (we recommend metal or PTFE pipes to reduce moisture ingress/waste outgassing)
  • Larger diameter pipe with converter for outlet hose (again, we recommend metal or PTFE)
  • KF vacuum feedthroughs for electrical supply (if necessary)

Moisture and Oxygen Sensors

The Ossila Glove Box comes with two sensor boards, one for the main chamber and one for the antechamber. These sensor boards each house an oxygen sensor and a H20 sensor that constantly monitor oxygen and moisture levels, as well as the internal chamber pressure and temperature. This information is recorded on the glove box hardware and can be copied to a computer via a USB connection.

Ossila Glove Box sensor board
The sensor board which measures O2 and H2O sensors and temperature sensors
Ossila Glove Box connector board
The associated connector board in the main glove box chamber

To fit the two sensors, simply align the legs on the sensor board with the holes on the connector board and push into place. Then, fix the sensor board to the connector board using the screws provided.

Individual sensors are required for the main chamber of the glove box and the antechamber. For the Ossila Glove Box, these are located on the right wall of the main glove box chamber and on the back wall of the antechamber.

Ossila glove box sensor board in the main chamber
Sensor board placement in the main chamber
Ossila glove box sensor board in the glove box antechamber
Sensor board placement in the antechamber

Glove Box Gloves

The Ossila Glove Box comes with two gloves, and 4 O-rings. The gloves are made of butyl rubber which has low moisture and O2 ingression. Use two O-rings per glove to fix them in place.

To attach the glove box gloves, pull the gloves over the two ports and secure them in place with two of the O-rings provided. These should be tight enough to hold the glove in place, ensuring a tight seal.

Fitting glove box gloves to the Ossila Glove Box: pull the gloves over the glove port
Pull the gloves round the glove box ports
Glove box gloves secured with O-rings
Secure the gloves using the O-rings provided

Make sure you check the gloves regularly for wear and tear and replace them as soon as possible if you find any holes. Small holes can be temporarily sealed using black electrical tape. However, this is not a permanent solution and you should replace the gloves as soon as possible.

Glove box glove hole
Small holes in the gloves can be temporarily sealed with black tape

Window Assembly

Glove box screens consist of multiple layers and seals to ensure that minimal leaks occur around the window edge. There are many different types of seal, but the Ossila Glove Box uses rubber gaskets to reduce leakage around the screen. These layers are secured into place using a metal frame.

To install the glove box screen, first position the rubber gasket on the glove box front by aligning the holes with the screws as below.

Glove box rubber gasket layer
Align the window layers with screws on the Glove Box front
Glove box rubber gasket layer fitted
First gasket layer placed on the Glove Box
Ossila glove box- use l-spacers to fit the glove box window
Place the L spacers on the bottom corners of the frame (see inset), then position the glass screen on top
Ossila glove box- fit the final rubber gasket layer
Place the final rubber gasket layer on top of the glass screen

Once the rubber interlayer is in position, use the L spacers to align the glass window onto the Glove Box front. Next, place the final rubber gasket on top, slightly overlapping with the window edge. This provides cushioning between the screen and the metal frame.

Ossila glove box- place the metal frame on the screen
The metal frame can be aligned in the same way as the other layers
Ossila glove box- fix the metal frame
Secure using bolts to create a tight seal

Once you have position all the gasket layers, place the metal frame on the glove box front and fix it into place using the domed nuts. First, tighten the domed nuts by hand, then secure with a wrench. Do not overtighten the nuts as this could cause damage to the glove box and compromise the seal.

Feedthrough Ports

The two ports on the left of the glove box chamber can allow electrical cable or vacuum feedthroughs into the glove box. Various KF-flange feedthrough adaptors can be used to allow power supply or vacuum lines into the glove box. You can also seal these ports using the blank KF-flange provided.

Ossila glove box - KF flange components
The individual components for a KF-flange blank seal
Ossila glove box - KF flange nipple
Feedthrough ports on left wall of the Ossila Glove Box

The blank KF-flange provided consists of a centring ring, a blank flange and a hinge clamp.

To seal the KF-flange, line up the KF-flange blanking plate in combination with the centring ring. Then place these on the flange nipple.

How to assemble to KF port seals
Ossila glove box - fitting blanking plate
The centring ring and blanking plate can be fitted into the port
Ossila glove box - fitting hinge clamp
Fix into place using the hinge clamp

Finally, secure the seal into place using the hinge clamp and tighten until a tight seal is formed around the port.

Inlet and Outlet Tubes

To insert the 12 mm inlet and outlet tubes, gently push the tubes into the correct feedthroughs into until they feel secure. We recommend using PTFE or metal pipes in order to decrease ingression of moisture/oxygen into the glove box, or waste gas into the lab environment from the outlet tube.

If the outlet tube needs to be more than 1 m long to reach the ventilated area, we recommend converting to a tube diameter of 16 mm. For lengths longer than 3 m, a wider diameter tube (>25 mm) will be required. A conversion pipe can be used to adapt the 12 mm outlet pipe, as shown below. To insert the outlet tube, connect the 12 mm pipe to the outlet feedthrough. The other end of this line should vent into a fume hood or ventilated area.

Ossila glove box - outlet bulkhead feedthrough
The lower feedthrough port
Ossila glove box - outlet pipe fitting
Converter feedthroughs can be used to connect to a larger diameter outlet tube if necessary

Similarly, to install the inlet tube, push the inlet tube into the upper bulkhead feedthrough. Connect this tube to a nitrogen supply which has been regulated to roughly 2 bar.

Ossila glove box - inlet bulkhead feedthrough
Push an inlet tube into the upper feedthrough
Ossila glove box - inlet pipe fitting
It is important to ensure the gas inlet is regulated to 2 bar

Pressure Relief Valve Assembly

Place the washer over the threaded end of the pressure relief valve and insert this end into the top corner of the glove box.

Pressure relief valve installation for the Ossila Glove Box
Installation Steps for the Pressure Relief Valve

Tighten the valve using a wrench. The bottom nut can be used to tighten the relief valve.

Starting the Glove Box

Once the glove box is completely sealed, simply plug into a wall socket, and turn on via the power switch on the back of the glove box.

Ossila glove box power source
Ossila Glove Box power source

The initial display is the Main Chamber Glove Box display page. This displays the oxygen and moisture levels and internal pressure within the main chamber of the glove box.

Glove Box Main Chamber Status Display
Glove Box Main Chamber Status Display

The first thing you need to do is set the time and date in the "Menu" section. This enables the Glove Box to accurately track O2 and H2O levels, temperature, and pressure. This can be useful for identifying problems within the glove box.

Ossila Glove Box Menu Display
Glove Box Menu Display

Next, you need to set the overpressure, O2 and H2O levels within the "Settings" section. We recommend that these are set for 1.5 mBar, 0.5% and 1% for Pressure, O2 and H2O levels respectively. Once defined, press "Start Purge" to start the initial purge.

Ossila glove box- setting page with inactive purge settings
Glove Box Settings Page: before automated purge has started
Ossila glove box- setting page with active purge settings
Glove Box Settings Page: after automated purge has started

You'll know the purge has begun when the button changes from "Inactive" (red) to "Active" (green). Additionally, the gloves will begin to inflate as the glove box aims to achieve the set overpressure.

Over the next few hours, the glove box will circulate nitrogen to reduce oxygen and moisture levels to below the desired levels. We recommend leaving the Glove Box for at least 12 hours in order to achieve a stable, inert environment. Once this is achieved the Glove Box is ready to use, and with regular maintenance, this environment can be sustained for an extended period of time.


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Glove Box

Laboratory Glove Box
  • Accurate Atmosphere Control
  • Cheap to Run
  • Automated Purging

From £8,500.00

Contributing Authors

  • Mary O'kane
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