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Micropipette Techniques for Accurate Measurements

Micropipette Techniques for Accurate Measurements

Correct technique is important to getting accurate measurements when using a micropipette. Follow the instructional videos below for guidance on measuring volumes accurately with a micropipette.

How to Change Micropipette Volume


To Change Micropipette Volume

  1. Hold the body of the micropipette in one hand and hold the plunger at rest position in the other hand.
  2. Twist the plunger clockwise to decrease the micropipette volume.
  3. Twist the plunger anticlockwise to increase the micropipette volume.

How to Dispense and Withdraw Liquid using a Micropipette


Dispensing Liquid using a Micropipette

Correct Technique to Dispense Liquid

  1. Place the micropipette tip vertically into the vial. Place the micropipette tip halfway into the vial and do not touch the sides of the vial.
  2. Press the plunger to the first stop, then pause.
  3. Press the plunger to the second stop to remove remaining liquid in the micropipette.
  4. Remove the micropipette tip from the vial.
  5. Release the plunger to rest position.

Incorrect Technique to Dispense Liquid

  1. Place the micropipette tip diagonally into the vial. Place the tip just below the top of the vial.
  2. Push the plunger to the first stop.
  3. Remove the micropipette tip from the vial and release the plunger to rest position.

Withdrawing Liquid using a Micropipette

Correct Technique to Withdraw Liquid

  1. Steadily push the plunger to the first stop.
  2. Place the micropipette tip vertically in the vial, halfway into the liquid. Do not touch the bottom or sides with the micropipette tip.
  3. Release the plunger to rest position to withdraw the liquid.
  4. Remove pipette tip from the vial slowly.

Incorrect Technique to Withdraw Liquid

  1. Forcefully push the plunger to the second stop.
  2. Place the micropipette tip diagonally in the vial, touching the bottom of the vial.
  3. Release the plunger to rest position to withdraw the liquid.
  4. Remove the micropipette from the vial.

Micropipette

Micropipette

Resources and Support


Micrpipette volume display Getting Started with a Micropipette

Using your micropipette correctly ensures the most accurate and reproducible measuring and dispensing of solutions.

Read more...
Micrpipette infographic Micropipette: Positioning and Withdrawing

Follow our guide to correctly position and withdraw liquid with your micropipette.

Read more...
Micrpipette infographic Micropipettes: Components and Parts

Micropipettes are indispensable tools in laboratories. These can be used in many applications ranging from thin film deposition by spin coating to cutting-edge molecular biology experiments like PCR (Polymerase Chain Reaction).

Read more...
Micrpipette infographic Dispensing Liquid from Micropipette

Micropipettes allow liquids to be measured and dispensed both precisely and quickly. Therefore, micropipettes are used in a wide range of applications from microbiology to analytical chemistry.

Read more...

Contributors


Written by

Amie Philpot

Scientific Writer

Diagram by

Sam Force

Graphic Designer

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