Syringe Pump User Manual

1. Overview

Syringe pumps are used in the field of scientific research to provide a reliable and repeatable method for moving liquids. Typically, the most common use of a syringe pump is to dispense a liquid at a known rate. This can be for use in processes such as pre-metered coating, automated systems, chemical synthesis, microfluidics, and flow chemistry. Other uses of syringe pumps include the withdrawal of liquids from a system for processes such as automated sample taking, and fluid level adjustment. By combining both infusion and withdrawal from syringes it is possible to use a syringe pump for an even wider array of applications including continuous flow of solutions, solution mixing, and the emulsification of solutions

A syringe pump works by fixing the barrel of the syringe to the body of the equipment while at the same time fixing the plunger to a moving platform. This movement is achieved via the use of a high accuracy stepper motor where the distance moved by the platform is well known and characterised. By knowing the internal diameter of the syringe being used it is possible to work out the volume displaced when the platform moves. The Ossila Syringe Pump provides you with a simple easily programmable system, where each program can have up to 99 different steps in which the rate, volume, and/or time of infusion/withdrawal can be chosen. By selecting the correct diameter syringe, it is possible to achieve flow rates as low as nanolitres per minute or as high as millilitres per second.

1.1 Applications

Solution dispensing
Solution mixing
Emulsification
Metered coatings
Solution quenching
Electrospinning
Electrospraying
Microfluidics
Flow chemistry

2. EU Declaration of Conformity (DoC)

Company Name: Ossila BV

Postal Address: Biopartner 3 Building, Galileiweg 8

Postcode: 2333 BD Leiden

Country: The Netherlands

Telephone Number: +31 (0)718 081020

Email Address: info@ossila.com

declare that the DoC is issued under our sole responsibility and belongs to the following product:

Product: Single Syringe Pump (L2003S1), Dual Syringe Pump (L2003D1)

Serial Number: L2003S1 - xxxx, L2003D1

Object of Declaration

Single Syringe Pump (L2003S1), Dual Syringe Pump (L2003D1)

The object of declaration described above is in conformity with the relevant Union harmonisation legislation:

Machinery Directive 2006/42/EC

EMC Directive 2014/30/EU

RoHS Directive 2011/65/EU

The folowing harmonised standards and technical specifications have been applied:

BS EN ISO 12100:2010 Safety of machinery - General principles for design - Risk assessment and risk reduction

Signed:

Name: Dr James Kingsley

Place: Leiden

Date: 16/11/2021

Декларация	за съответствие на ЕС
Производител	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Декларира с цялата си отговорност, че посоченото оборудване съответства на приложимото законодателство на ЕС за хармонизиране, посочено на предходната(-ите) страница(-и) на настоящия документ.

[Čeština]	Prohlášení o shodě EU
Výrobce	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Prohlašujeme na vlastní odpovědnost, že uvedené zařízeni je v souladu s příslušnými harmonizačními předpisy EU uvedenými na předchozích stranách tohoto dokumentu.

[Dansk]	EU-overensstemme lseserklærin g
Producent	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Erklærer herved, at vi alene er ansvarlige for, at det nævnte udstyr er i overensstemmelse med den relevante EUharmoniseringslovgivning, der er anført på den/de foregående side(r) i dette dokument.

[Deutsch]	EU-Konformitätserklärung
Hersteller	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Wir erklären in alleiniger Verantwortung, dass das aufgeführte Gerät konform mit der relevanten EUHarmonisierungsgesetzgebung auf den vorangegangenen Seiten dieses Dokuments ist.

[Eesti keel]	ELi vastavusavaldus
Tootja	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Kinnitame oma ainuvastutusel, et loetletud seadmed on kooskõlas antud dokumendi eelmisel lehelküljel / eelmistel lehekülgedel ära toodud asjaomaste ELi ühtlustamise õigusaktidega.

[Ελληνικά]	Δήλωση πιστότητας ΕΕ
Κατασκευαστής	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Δηλώνουμε υπεύθυνα όn ο αναφερόμενος εξοπλισμός συμμορφώνεται με τη σχεnκή νομοθεσία εναρμόνισης της ΕΕ που υπάρχει σnς προηγούμενες σελίδες του παρόντος εγγράφου.

[Español]	Declaración de conformidad UE
Fabricante	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Declaramos bajo nuestra única responsabilidad que el siguiente producto se ajusta a la pertinente legislación de armonización de la UE enumerada en las páginas anteriores de este documento.

[Français]	Déclaration de conformité UE
Fabricant	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Déclarons sous notre seule responsabilité que le matériel mentionné est conforme à la législation en vigueur de l'UE présentée sur la/les page(s) précédente(s) de ce document.

[Hrvatski]	E.U izjava o sukladnosti
Proizvođač	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Izjavljujemo na vlastitu odgovornost da je navedena oprema sukladna s mjerodavnim zakonodavstvom EU-a o usklađivanju koje je navedeno na prethodnoj(nim) stranici(ama) ovoga dokumenta.

[Italiano]	Dichiarazione di conformità UE
Produttore	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Si dichiara sotto la propria personale responsabilità che l'apparecchiatura in elenco è conforme alla normativa di armonizzazione UE rilevante indicata nelle pagine precedenti del presente documento.

[Latviešu]	ES atbils tības deklarācija
Ražotājs	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Ar pilnu atbilclību paziņojam, ka uzskaitītais aprīkojums atbilst attiecīgajiem ES saskaņošanas tiesību aktiem, kas minēti iepriekšējās šī dokumenta lapās.

[Lietuvių k.]	ES atitikties deklaracija
Gamintojas	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
atsakingai pareiškia, kad išvardinta įranga atitinka aktualius ES harmonizavimo teisės aktus, nurodytus ankstesniuose šio dokumento

[Magyar]	EU-s megfelelőségi nyilatkozat
Gyártó	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Kizárólagos felelösségünk mellett kijelentjük, hogy a felsorolt eszköz megfelel az ezen dokumentum előző oldalán/oldalain található EU-s összehangolt jogszabályok vonatkozó rendelkezéseinek.

[Nederlands]	EU-Conformiteitsverklaring
Fabrikant	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Verklaart onder onze uitsluitende verantwoordelijkheid dat de vermelde apparatuur in overeenstemming is met de relevante harmonisatiewetgeving van de EU op de vorige pagina('s) van dit document.

[Norsk]	EU-samsvarserklæ ring
Produsent	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Erklærer under vårt eneansvar at utstyret oppført er i overholdelse med relevant EU-harmoniseringslavverk som står på de(n) forrige siden(e) i dette dokumentet.

[Polski]	Deklaracja zgodności Unii Europejskiej
Producent	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Oświadczamy na własną odpowiedzialność, że podane urządzenie jest zgodne ze stosownymi przepisami harmonizacyjnymi Unii Europejskiej, które przedstawiono na poprzednich stronach niniejszego dokumentu.

[Por tuguês]	Declaração de Conformidade UE
Fabricante	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Declara sob sua exclusiva responsabilidade que o equipamento indicado está em conformidade com a legislação de harmonização relevante da UE mencionada na(s) página(s) anterior(es) deste documento.

[Română]	Declaraţie de conformitate UE
Producător	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Declară pe proprie răspundere că echipamentul prezentat este în conformitate cu prevederile legislaţiei UE de armonizare aplicabile prezentate la pagina/paginile anterioare a/ale acestui document.

[Slovensky]	Vyhlásenie o zhode pre EÚ
Výrobca	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Na vlastnú zodpovednosť prehlasuje, že uvedené zariadenie je v súlade s príslušnými právnymi predpismi EÚ o harmonizácii uvedenými na predchádzajúcich stranách tohto dokumentu.

[Slovenščina]	Izjava EU o skladnosti
Proizvajalec	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
s polno odgovornostjo izjavlja, da je navedena oprema skladna z veljavno uskladitveno zakonodajo EU, navedeno na prejšnji strani/prejšnjih straneh tega dokumenta.

[Suomi]	EU-vaatimustenm ukaisuusvakuutus
Valmistaja	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Vakuutamme täten olevamme yksin vastuussa siitä, että tässä asiakirjassa luetellut laitteet ovat tämän asiakirjan sivuilla edellisillä sivuilla kuvattujen olennaisten yhdenmukaistamista koskevien EU-säädösten vaatimusten mukaisia.

[Svenska]	EU-försäkran om överensstämmelse
Tillverkare	Ossila BV, Biopartner 3 building, Galileiweg 8, 2333 BD Leiden, NL.
Vi intygar härmed att den utrustning som förtecknas överensstämmer med relevanta förordningar gällande EUharmonisering som fmns på föregående

3. Safety

3.1 Warning

Pinch points present when in operation, keep hands clear of moving stage.

3.2 Use of Equipment

CAUTION: For research use only. Not for clinical use on patients.

The Ossila Syringe Pump is designed to be used as instructed, it is intended to be used under the following conditions:

Indoors in a laboratory environment (pollution degree 2).
Altitudes up to 2000 m.
Temperatures of 5°C to 40°C; maximum relative humidity of 80% up to 31°C.

The Syringe Pump is supplied with a 24V/2A power adapter with a power cord for the country of purchase, in accordance with European Commission regulations and British Standards. Use of any other electrical power cables, adaptors or transformers is not recommended.

3.3 Hazard Icons

The symbols shown in Table 3.1 can be found at points throughout the manual. Note each warning before attempting any associated operations.

Table 3.1 Hazard warning labels used in this manual.

Symbol	Associated Hazard
	General warning or caution, which accompanying text will explain
	Electrical shock
	Pinch point or entanglement hazard

3.4 General Hazards

Before installing or operating the Syringe Pump, there are several health and safety precautions which must be followed and executed to ensure safe installation and operation.

WARNING: Improper handling when operating or servicing this equipment can result in serious injury. Read this manual before operating or servicing this equipment.

CAUTION: During operation, the movement of the plunger clamp and the rotation of the lead screw/coupler could result in a pinch point hazard and/or an entanglement hazard. Caution should be taken whenever the system is in operation.

3.5 Power Cord Safety

Emergency power disconnect options: Use the power cord as a disconnecting method and remove it from the power source. To facilitate disconnect, make sure the power outlet for this cord is readily accessible to the operator.

3.6 Servicing

If servicing is required, please return the unit to Ossila Ltd The warranty will be invalidated if:

Modification or service has taken place by anyone other than an Ossila engineer.
The unit has been subjected to chemical damage through improper use.
The unit has been operated outside the usage parameters stated in the user documentation associated with the unit.
The unit has been rendered inoperable through accident, misuse, contamination, improper maintenance, modification, or other external causes.

3.7 Health and Safety - Operation

Pinch point and entanglement hazards are present during operation of the syringe pump. As some precaution users should avoid handling or leaning over the equipment during operation in order to avoid possible crushing or entanglement of hair and/or clothing.

3.8 Health and Safety - Servicing

Service or installation work that includes integrating electrical components should be performed by an Ossila engineer only. Never alter the wiring of any purchased equipment. If changes are made, such alterations may damage the equipment, cause injury or death. At the very least, such alterations may void your equipment’s warranty.

4. Equipment Purpose

The Ossila Syringe Pump has been designed to provide users with a tool to move volumes of fluids accurately and repeatedly at specified rates. The equipment can be used in a wide variety of experiments where accurate dispense volumes and/or dispense rates are required. In order to achieve this, the system uses a stepper motor to enable movement of specified distances. This is done by securing the body of the syringe to the frame of the syringe pump while at the same time securing the syringe plunger to the moving holder. The internal diameter of the syringe can be used to determine the volume displaced by the movement of the stepper motor and plunger. By choosing the appropriate syringe size, a wide range of dispense rates can be achieved.

The exact syringe types, deposition rates, deposition volumes, and solutions used are up to the user to choose and will vary depending upon the type of experiment being undertaken. For more information on uses please see the operating instructions and guidelines, for more information on the dispensing limits please see the specifications.

5. Unpacking

5.1 Packing List

The standard items included with the Syringe Pump are:

The Syringe Pump unit
Universal power adapter

5.2 Damage Inspection

Examine the components for evidence of shipping damage, if damage has occurred, please contact Ossila directly for further action. The shipping packaging will come with a shock indicator to show if there has been any mishandling of the package during transportation

6. Specifications

The specifications for the syringe pump software are shown in Table 6.1, and the hardware specifications are shown in Table 6.2.

Table 6.1. Ossila Syringe Pump software specifications

Number of Programmes	10
Steps per Programme	99
Syringe Control	Independent Control
Syringe Size Settings	5 pre-sets, 1 customisable diameter
Force Tuning	5% to 100% (5% increments)
Minimum Syringe Size	0.5μl
Memory	Non-Volatile Memory

Table 6.2. Ossila Syringe Pump hardware specifications

Syringes	1 (L2003S1); 2(L2003D1)
Operation mode	Infusion and withdrawal
Rate accuracy	±3%
Drive motor	1.8° Stepper motor
Max linear force	500N at 100% force
Motor drive controller	Microprocessor with 1/64 microstepping
Number of microsteps per revolution	12,800
Screw pitch	1mm
Distance per microstep	78nm
Maximum step rate/distance	27 μstep.min^-1; 5mm.s^-1
Minimum step rate/distance	27sec.μstep^-1; 12μm.s^-1
Maximum syringe size	50 (60)ml
Safety	Limit switches; crash sensing
Power	24 V DC; 2.0 A
Power supply Input voltage range	100 - 240VAC; 50/60Hz; 50VA
Operating temperature/humidity	5°C to 40°C; Up to 80% RH @ 31°C
Overall dimensions	Width: 330mm Height: 140mm Depth: 260mm

7. System Components

The Syringe Pump (L2003S1/L2003D1) comprises two items:

Syringe Pump unit (Figure 7.1)
24 V mains power supply adapter (Figure 7.2)

Ossila Syringe Pump Schematic — Figure 7.1 Syringe Pump

Schematic of 24V mains power supply adapter — Figure 7.2 24V mains power supply adapter

The Syringe Pump unit is powered from a 24 V, 2 A mains power supply adapter. This low voltage supply is convenient and reduces the health and safety risks involved.

8. Installation

Place the unit on a solid, level work surface.

Ensure the area is free from vibrations, temperature extremes and highly flammable or explosive materials.

Before plugging in the Syringe Pump, ensure the power switch on the unit is switched to the ‘0’ position (off).
Connect the power supply first to the mains outlet and second to the Syringe Pump unit.

See Figure 8.1 for connecting the Syringe Pump to the power supply cable.

Set the Syringe Pump power switch to the ‘1’ position to turn on.

Syringe pump with power supply connected — Figure 8.1 Installation of th Syringe Pump by plugging in the power supply cable

9. Operation

9.1 Syringe Pump Schematic

A top-down view of the Syringe Pump is shown in Figure 9.1 with all the relevant components highlighted.

Labelled diagram of the Ossila Syringe Pump — Figure 9.1 Syringe Pump schematic

9.2 Attaching a Syringe to the System

When using the Syringe Pump the following procedures should be done while attaching the syringe to the system to achieve the best results.

The solution should be preloaded into the syringe; make sure all air bubbles are removed from the syringe before attaching to the system.
The syringe barrel should be placed flat into the groove on the syringe holder, and the barrel top should be placed inside the clamping mechanism (see Figure. 9.2). The top clamping arm should be lowered onto the top of the syringe and locked into place by tightening the wing nut on the side of the syringe holder. The barrel top should be clamped into place by tightening the wing nut screws as the opposite end of the syringe holder.

Syringe placed into the groove of the syringe clamping mechanism — Figure 9.2 Placement of the syringe into the groove along with the barrel top placed in the clamping mechanism

Syringe in holder with top clamping arm used to secure the syringe — Figure 9.3 Placement of the top clamping arm

Fixing the barrel using the clamp and screws (side view) — Figure 9.4 Fixing of the barrel top into clamping mechanism

Enter manual mode on the syringe and drive the plunger holder till it rests against the end of the plunger. Slide the end of the plunger into the clamping mechanism and tighten, making sure it is parallel with the barrel of the syringe.

Plunger being secured into the clamping mechanism — Figure 9.5 Plunger clamping mechanism

Connect any additional tubing or fixtures required for your experiment. Using the manual mode preload the fixtures and tubing with your solution.

9.3 User Interface

Figure 9.6 Syringe Pump LCD screen and keypad

Table 9.1. Operational buttons and their associated functions

Button	Function
S (Settings)	Enters the Settings menu where syringe diameter, and maximum force can be set
M (Manual)	Enters Manual mode where the plunger holder can be moved
P (Program)	Enters the Program mode where saved programs can be selected and/or edited
	Navigates ‘Up’ through menus; increases selected values by 1; increases unit size
	Navigates ‘Down’ through menus; decreases selected values by 1; decreases unit size
	Navigates ‘Right’ through menus
	Navigates ‘Left’ through menus
	Press to select, edit, or accept changes
	Stop current running program
	Run currently selected program

9.4 Program Operation

9.4.1 MODEL: L2003S1 (Single Syringe Pump)

WARNING! Pinch points and entanglement hazards!

The rotation of the lead screw/coupler and the movement of the plunger holder present entanglement and pinch hazards. Care should be taken when the system is running.

Turn the Ossila Syringe Pump power switch on (position ‘1’); the bootup screen will show as below.
The system automatically goes to Settings mode screen after the bootup screen. To set the syringe diameter enter the settings menu by pressing the “S” Button, navigate to syringe selection (as shown in the image below). Press “OK” to select what diameter of syringe to choose.
For syringes purchased from Ossila there are several pre-set diameters for quick selection, these are 1ml, 2ml, 5ml, 10ml, and 50ml. To change the syringe size setting, Press “OK” and navigate the syringe number by pressing “Left” or “Right” buttons.
For custom diameters navigate to custom and press “OK” to edit. To edit the diameter, highlight diameter and press “OK”. Use “Up” or “Down” and “Left” or “Right” buttons to set the internal diameter and press “OK” to set the value. Highlight the volume and press “OK” to edit.
A warning will appear if the diameter is not set for a custom syringe.
A warning will appear if the volume is not set for a custom syringe.
To change the force setting value for the syringe, navigate to force and press “OK” to edit. To change the value, press “Up” or “Down” buttons for increment/decrement value of 5.
To move the plunger holder without setting a program press the “M” button, this will take you to Manual mode (as shown in the image below). Press “Left” or “Right” buttons to move the plunger holder towards the coupler or towards the syringe clamp. Press “OK” to cycle through pre-set speeds.
To set a program to run automatically go to the program menu by pressing the “P” button, select the program you wish to edit and run by navigating to the program number (as shown in the image below) and pressing “OK”. Pressing “Up” or “Down” buttons will allow you to switch between pre-saved programs.
To change the program number, press the “Up” button until the program number highlighted and press “Left” or “Right” buttons to change the program number.
To edit the number of steps, navigate to the steps line and press “OK” to edit and “Up” or “Down” buttons to increase or decrease the value. When finished press “OK” again to save the changes.
To change the step number, press “Left” or “Right” buttons to cycle through the different steps.
To edit the dispense rate navigate to the dispense rate line and press “OK” to begin editing. Use “Left” or “Right” buttons to highlight the number you wish to edit and “Up” or “Down” buttons to increase or decrease the value. When finished press “OK” again to save the changes. Note: The dispense time for the step will automatically update to account for the new rate.
Navigate to the unit to change the dispense rate unit by pressing “Up” or “Down” buttons to change from microliter (μl) to millilitre (ml) or vice versa.
A warning will appear if the set rate is higher than allowed for the syringe size. The value will be recalculated and set to max rate for the particular syringe.
To edit the dispense volume navigate to the dispense volume line and press “OK” to begin editing. Use “Left” or “Right” buttons to highlight the number you wish to edit and “Up” or “Down” buttons to increase or decrease the value. When finished press “OK” again to save the changes. Note: The dispense time for the step will automatically update to account for the new rate.
Navigate to the unit to change the dispense volume unit by pressing “Up” or “Down” buttons to change from microliter (μl) to millilitre (ml) or vice versa.
A warning will appear if the set volume higher than allow for the syringe size. The value will be recalculated and set to max volume for the syringe.
The user can either dispense or infuse the solution using Ossila Syringe Pump by navigating to volume sign (+), and press “Up” or “Down” buttons to change the sign. The ‘+’ sign indicates dispensing and ‘–’ indicates infusing.
The user can edit the time by navigating to dispense time and pressing “OK” to edit. The format of time is HH:MM:SS (H – Hour, M – Minute, S – Second). The dispense volume for the step will automatically update to account for the new dispense time setting. A warning will appear if the volume is larger than allowed for the syringe size. The value will be recalculated to the correct volume for the syringe.
The user can run the program by pressing the “Start” button. The running program page will then appear to indicate this.
Once the dispensing is complete, a message with pop up to notify the user.
The user can stop the operation by pressing the “Stop” button and the warning will appear as follows.
The Ossila Syringe Pump is equipped with a safety warning. The System temperature is monitored while the system is On. In the case of high temperatures, a warning will appear to notify the user.

9.4.2 MODEL: L2003D1 (Dual Syringe Pump)

WARNING! Pinch points and entanglement hazards!

The rotation of the lead screw/coupler and the movement of the plunger holder present entanglement and pinch hazards. Care should be taken when the system is running.

Turn the Ossila Syringe Pump power switch on (position ‘1’); the bootup screen will show as below.
To set the syringe diameter enter the Settings menu by pressing the “S” button, navigate to syringe selection (as shown in the image below). Press “OK” to select what diameter of syringe to choose.
For syringes purchased from Ossila there are several pre-set diameters for quick selection. These include 1ml, 2ml, 5ml, 10ml, and 50ml.
For custom diameters navigate to custom and press “OK” to edit. To edit the diameter, highlight diameter and press “OK” use “Up” or “Down” and “Left” or “Right” buttons to set the internal diameter and press “OK” to set the value. Highlight the volume and press “OK” to edit.
A warning will appear if the diameter is not set for custom syringe.
A warning will appear if the volume is not set for custom syringe.
To change the force setting value for the syringe, navigate to force and press “OK” to edit. The change the value, press “Up” or “Down” buttons for increment/decrement value of 5.
To move the plunger holder without setting a program press the “M” button. This will take you to Manual mode (as shown in the image below). Press “Up” or “Down” buttons to select the syringe you want to move. Press “Left” or “Right” buttons to move the plunger holder towards the coupler or towards the syringe clamp. Press “OK” to cycle through pre-set speeds for selected syringe.
To set a Program to run automatically go to the program menu by pressing the “P” button. Select the program you wish to edit and run by navigating to the program number (as shown in the image below) and press “OK”. Pressing “Up” or “Down” buttons will allow you to switch between pre-saved programs.
The program can be edited by changing the number of steps, the dispense rate for each step, and the dispense volume for each step.
To edit the number of steps, navigate to the steps line and press “OK” to edit use “Left” or “Right” buttons to highlight the number you wish to edit and “Up” or “Down” buttons to increase or decrease the value. When finished press “OK” again to save the changes.
To change the step number you are on, navigate to the steps line and press “Left” or “Right” buttons to cycle through the different steps.
To edit the dispense rate navigate to the dispense rate line and press “OK” to begin editing. Use “Left” or “Right” buttons to highlight the number you wish to edit and “Up” or “Down” buttons to increase or decrease the value. When finished press “OK” again to save the changes The dispense time for the step will automatically update to account for the new rate.
Edit the dispense rate by pressing “Up” or “Down” buttons to change the units from microliter (μl) to millilitre (ml) or vice versa.
A warning will appear if the set rate higher than allow for the syringe size. The value will be recalculated and set to max rate for the particular syringe.
To adjust the dispense volume navigate to the dispense volume line and press “OK” to begin editing. Use “Left” or “Right” buttons to highlight the number you wish to edit and “Up” or “Down” buttons to increase or decrease the value. When finished press “OK” again to save the changes. The dispense time for the step will automatically update to account for the new rate.
Change the dispense volume unit by pressing “Up” or “Down” buttons to switch from microliter (μl) to millilitre (ml) or vice versa.
A warning will appear if the set volume is larger than the syringe size can allow. The value will be recalculated and set to the max volume for the syringe.
The user can either dispense or infuse the solution using Ossila Syringe Pump by navigating to volume sign (+), and press “Up” or “Down” buttons to change the sign. The ‘+’ sign indicates dispensing and ‘–‘ indicates infusing.
The user can edit the time by navigating to the dispense time and pressing “OK” to edit. The format of time is HH:MM:SS (H – Hour, M – Minute, S – Second). The dispense volume for the step will automatically update to account for the new dispense time setting. A warning will appear if the volume is larger than the syringe size allows. The value will be recalculated to the correct volume for the syringe.
To edit the parameter for syringe 2 (S2), navigate the cursor down by pressing “Up” or “Down” buttons and press “OK” to edit an individual parameter value.
The user can run the program by pressing the “Start” button. The running program page will appear to indicate the program is running.
Both syringes are dispensing
Syringe 1 is dispensing and syringe 2 is infusing
Syringe 1 is infusing and syringe 2 is dispensing
Both syringes are infusing
Once the timer is finished, a warning will appear to alert the suer that dispensing is complete.
If the one of the syringes completes dispensing, the “complete” message will pop up to notify the user.
While the system is running, the user can stop one of the syringes by pressing the “Up” or “Down” buttons. Press “OK” to stop the selected syringe.
If both syringes are stopped by the user, the message will appear as follow.
To stop both syringes simultaneously, press the “Stop” button. The message will appear as follow.
The Ossila Syringe Pump is equipped with a safety warning. The System temperature is monitored while the system is On. In the case of high temperatures, a warning will appear to notify the user.
The system is also equipped with safety switches in case of software malfunction. There are two switches to safeguard forward and reverse movement. In the event of either switch being pressed, the following warning will appear:

10. Maintenance

The rail guide and the lead screw should be sparingly lubricated periodically; every 200 hrs of usage. The rail guide and the lead screw should be lubricated with super lube synthetic grease provided with the pump.

To clean the exterior surfaces, use a lint-free cloth to remove loose dust. Use care to avoid scratching the clear display window. For more efficient cleaning, use a soft cloth dampened (not soaked) with water, an aqueous solution of 75% isopropyl alcohol, or a mild detergent.

11. Troubleshooting

Table 11.1. Troubleshooting guidelines for the Ossila Syringe Pump

Problem	Possible Cause	Action
No power/display	The power switch on the unit is in the OFF position.	Check the connection and ensure the power is turned ON.
	The power supply may not be connected properly.	Ensure the unit is firmly plugged in to the power supply and the plug is firmly connected to both the adapter and the working power socket.
	The fuse on the rear panel has blown.	Ensure the unit is unplugged. Check the fuse on the rear panel. If it has blown, replace with a suitably rated 1A slow blow fuse.
	If the unit was supplied with a transformer, it may be faulty.	Contact Ossila for a replacement transformer.
	Fault on circuit board.	If all the above causes have been considered, there may be a fault on the control board. Please contact Ossila for information
Motor Stalled (Syringe Pump)	Syringe has hit first safety switch	Syringe plunger hitting the bottom / a kink in the tubing (occlusion) / Syringe plunger binding / Low force setting
Crash Warning	The secondary safety switch has accidentally been triggered	Turn the unit off and on again to see if the warning remains
	The syringe holder is triggering the secondary safety switch	Rotate the lead screw by hand to move the syringe holder closer to the centre of travel
	Internal fault in the secondary safety switch	If all the above has been considered there may be a fault with the secondary safety switch; contact Ossila for more information

Syringe Pump User Manual

Contents

1. Overview

1.1 Applications

2. EU Declaration of Conformity (DoC)

3. Safety

3.1 Warning

3.2 Use of Equipment

3.3 Hazard Icons

3.4 General Hazards

3.5 Power Cord Safety

3.6 Servicing

3.7 Health and Safety - Operation

3.8 Health and Safety - Servicing

4. Equipment Purpose

5. Unpacking

5.1 Packing List

5.2 Damage Inspection

6. Specifications

7. System Components

8. Installation

9. Operation

9.1 Syringe Pump Schematic

9.2 Attaching a Syringe to the System

9.3 User Interface

9.4 Program Operation

9.4.1 MODEL: L2003S1 (Single Syringe Pump)

9.4.2 MODEL: L2003D1 (Dual Syringe Pump)

10. Maintenance

11. Troubleshooting