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Choosing A Light Source For Your Spectrometer

Choosing A Light Source For Your Spectrometer

Most spectroscopy experiments require at least one light source. Electroluminescence measurements use an external current as an excitation source. However, most characterization of an optical material requires some sort of incident light. 

  • Photoluminescence and fluorescence measurements require a high-energy monochromatic light sources, often in the UV range.
  • Monochromatic light sources can be useful for absorbance spectroscopy when measuring optical density. These measurements are often standardized to a specific wavelength. 
  • Absorbance measurements require illumination over a broad wavelength range, so require a broadband light source such as the Ossila LED Light Source.
  • A tunable light source provides an adaptable combination of monochromatic and broadband light sources. These light sources can cycle through a range of wavelengths to build a broadband spectrum, or can be used to isolate individual wavelengths of light.  
Tunable light source for flexible spectroscopy

There are multiple other factors to consider when choosing light sources for an spectroscopy lab. Choosing the best light source depends on:

  • Type of measurement or experiment
  • Specifications of your spectrometer or spectrophotometer 
  • Compatibility with other spectroscopy components (i.e. rail based systems, optical fiber compatibility, etc)
  • Budget
  • Safety restrictions
  • Available set-up and maintenance time 

You will likely need different light sources for different optical measurements. Therefore, it is quite likely that you will need more than one light source in order to fully characterize your organic electronic materials.

 

Comparing Different Spectroscopy Light Sources


In the table below, we have done a brief comparison of different light sources. Here, we have outlined their ideal applications, costs and other important information.

 

White Light LEDs Tungsten Halogen + Deuterium Light Sources Monochromatic LEDs Lasers Tunable Light Source
Type Broadband Broadband Monochromatic Monochromatic Monochromatic
Suitable measurements
  • Absorbance measurements
  • Transmission measurements
  • Reflectivity measurements
  • Absorbance measurements
  • Transmission measurements
  • Reflectivity measurements
  • Photoluminescence
  • Optical Density
  • Measuring concentration of certain molecule
    • Photoluminescence / Steady State Fluorescence
    • ASE
    • Time-resolved spectroscopy (for pulsed laser systems)
    • Absorbance
    • Photoluminescence
    • Fluorescence
    • Transmission
    • Excitation / Emission Spectra
    Advantages Long bulb lifetimes Wide spectral range Long bulb lifetimes High power (for weak fluorescence), can emit short pulses for TRPL High versatility
    Cost Low High Low Mid-range to High Mid-range to High
    Emission Spectra

    Broad spectrum

     (360 nm – 900 nm for Ossila LED Light Source)

    Very broad spectrum (190 nm – 2200 nm)

    Well-defined narrowband emission

    (e.g. 370 nm for Ossila USB UV Light Source - 700 nm for the Ossila USB Infrared Light Source)

    Well-defined emissions (can be tunable depending on the laser)

    Narrow emission but over a wide wavelength range

    (Ossila Tunable LED Light Source can select a wavelength 380-1000 nm with < 1 nm accuracy)

    Warm-Up Time Minimal 30 minutes – 1 hour Minimal 5–60 minutes (will vary depending on laser material and mode) Minimal
    Temperature Control Needed? Not needed for small LEDs, larger LEDs often have integrated cooling fans Yes – to stop overheating Not needed for small LEDs, larger LEDs often have integrated cooling fans Most likely – but depends on the laser Not needed for small LEDs, larger LEDs often have integrated cooling fans
    Set Up and Calibration Time Low Low Low High Low
    Safety Considerations Low Low Low–Medium High Low

    In order to take the broadest range of general spectroscopy measurements, you will need multiple light sources, such as can be found in our LED Light Source Set or the Tunable LED Light Source.

    Ossila Light Sources


    Ossila sell many low-cost light sources which are compatible with the Ossila USB Spectrometer, but also with other spectroscopy equipment via the use of standard optical fibers. This will enable you to take a range of spectroscopy measurements quickly and easily.

    Tunable LED Light Source

    Tunable LED Light Source

    The Tunable LED Light Source combines a monochromator with a broadband LED light source, to emit light with a narrow spectral bandwidth at wavelengths between 380-1000 nm with < 1nm accuracy. The tunable light source can be used as a monochromatic excitation source for fluorescence emission experiments, and can also scan through the full wavelength range to measure absorbance and transmission spectra. 

    Ideal for:

    • Recording absorbance & transmission spectra, with high accuracy,
    • Compatible with optical rail based systems
    Broadband White Light Source

    LED Light Source

    Our LED Light Source outputs light over the visible spectrum (380 nm - 900 nm) with a relatively smooth emission spectrum, compared to other white light LEDs. This white light source is considerably smaller than tungsten/deuterium sources with lower warm up time and longer bulb lifetime.

    Ideal for:

    • Absorbance, transmission and reflectivity measurements
    • Compatible with optical rail based systems
      UV Light Source

      UV Light Source

      Our USB-C powered USB UV Light Source emits light at 370 nm. It will not be as powerful as laser excitation but is considerably safer.

      Ideal  for:

      • Optical excitation in visible fluorescence spectroscopy measurements.
      • Compatible with modular and optical fiber based systems
      Infrared Light Source

      Infrared Light Source

      This USB-C powered USB Infrared Light Source emits light at specific infrared wavelengths. Infrared radiation is lower intensity than other excitation sources so will minimize damage to more sensitive samples. You can choose from range of wavelengths between 700 - 980 nm. It will not be as powerful as laser excitation but is considerably safer and less expensive.

      Ideal for:

      • Studying biological samples, functional groups, chemical bonds and molecular Identification
      • Compatible with modular and optical fiber based systems
      Broadband White Light Source

      Broadband White Light Source

      Our USB Broadband White Light Source outputs light over the entire visible spectrum (360 nm - 900 nm).

      Ideal for:

      • Absorbance, transmission and reflectivity measurements
      • Compatible with modular and optical fiber based systems
      UV Light Source

      LED Light Source

      Our range of USB-C powered USB LED Light Source emit light centered around a single wavelength for specific excitation. You can choose from a range of specific wavelengths between 375-980 nm. This can be useful for

      Suitable for:

      • Fluorescence excitation sources & for optical density measurements
      • Compatible with modular and optical fiber based systems
      Represented output of Ossila LED Light Sources
      Normalized spectra of our various LED light sources

      In combination, these two light sources will provide you with everything you need to take basic spectroscopy measurements. These are compatible with standard spectroscopy fittings (SMA 905 fiber connections, M6 optical breadboard holes) so that they can be easily integrated with any spectroscopy system.

      LED Light Sources Set

      LED Light Sources Set

      Learn More


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      Contributing Authors


      Written by

      Dr. Mary O'Kane

      Application Scientist

       

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