Choosing A Light Source For Your Spectrometer

Most spectroscopy experiments require at least one light source. Electroluminescence measurements use an external current as an excitation source. However, most characterization of an optical material requires some sort of incident light.
- Photoluminescence and fluorescence measurements require a high-energy monochromatic light sources, often in the UV range.
- Monochromatic light sources can be useful for absorbance spectroscopy when measuring optical density. These measurements are often standardized to a specific wavelength.
- Absorbance measurements require illumination over a broad wavelength range, so require a broadband light source such as the Ossila LED Light Source.
- A tunable light source provides an adaptable combination of monochromatic and broadband light sources. These light sources can cycle through a range of wavelengths to build a broadband spectrum, or can be used to isolate individual wavelengths of light.

There are multiple other factors to consider when choosing light sources for an spectroscopy lab. Choosing the best light source depends on:
- Type of measurement or experiment
- Specifications of your spectrometer or spectrophotometer
- Compatibility with other spectroscopy components (i.e. rail based systems, optical fiber compatibility, etc)
- Budget
- Safety restrictions
- Available set-up and maintenance time
You will likely need different light sources for different optical measurements. Therefore, it is quite likely that you will need more than one light source in order to fully characterize your organic electronic materials.
Comparing Different Spectroscopy Light Sources
In the table below, we have done a brief comparison of different light sources. Here, we have outlined their ideal applications, costs and other important information.
| White Light LEDs | Tungsten Halogen + Deuterium Light Sources | Monochromatic LEDs | Lasers | Tunable Light Source | |
|---|---|---|---|---|---|
| Type | Broadband | Broadband | Monochromatic | Monochromatic | Monochromatic |
| Suitable measurements |
|
|
|
|
|
| Advantages | Long bulb lifetimes | Wide spectral range | Long bulb lifetimes | High power (for weak fluorescence), can emit short pulses for TRPL | High versatility |
| Cost | Low | High | Low | Mid-range to High | Mid-range to High |
| Emission Spectra |
Broad spectrum (360 nm – 900 nm for Ossila LED Light Source) |
Very broad spectrum (190 nm – 2200 nm) |
Well-defined narrowband emission (e.g. 370 nm for Ossila USB UV Light Source - 700 nm for the Ossila USB Infrared Light Source) |
Well-defined emissions (can be tunable depending on the laser) |
Narrow emission but over a wide wavelength range (Ossila Tunable LED Light Source can select a wavelength 380-1000 nm with < 1 nm accuracy) |
| Warm-Up Time | Minimal | 30 minutes – 1 hour | Minimal | 5–60 minutes (will vary depending on laser material and mode) | Minimal |
| Temperature Control Needed? | Not needed for small LEDs, larger LEDs often have integrated cooling fans | Yes – to stop overheating | Not needed for small LEDs, larger LEDs often have integrated cooling fans | Most likely – but depends on the laser | Not needed for small LEDs, larger LEDs often have integrated cooling fans |
| Set Up and Calibration Time | Low | Low | Low | High | Low |
| Safety Considerations | Low | Low | Low–Medium | High | Low |
In order to take the broadest range of general spectroscopy measurements, you will need multiple light sources, such as can be found in our LED Light Source Set or the Tunable LED Light Source.
Ossila Light Sources
Ossila sell many low-cost light sources which are compatible with the Ossila USB Spectrometer, but also with other spectroscopy equipment via the use of standard optical fibers. This will enable you to take a range of spectroscopy measurements quickly and easily.
Tunable LED Light Source
The Tunable LED Light Source combines a monochromator with a broadband LED light source, to emit light with a narrow spectral bandwidth at wavelengths between 380-1000 nm with < 1nm accuracy. The tunable light source can be used as a monochromatic excitation source for fluorescence emission experiments, and can also scan through the full wavelength range to measure absorbance and transmission spectra.
Ideal for:
- Recording absorbance & transmission spectra, with high accuracy,
- Compatible with optical rail based systems
LED Light Source
Our LED Light Source outputs light over the visible spectrum (380 nm - 900 nm) with a relatively smooth emission spectrum, compared to other white light LEDs. This white light source is considerably smaller than tungsten/deuterium sources with lower warm up time and longer bulb lifetime.
Ideal for:
- Absorbance, transmission and reflectivity measurements
- Compatible with optical rail based systems
UV Light Source
Our USB-C powered USB UV Light Source emits light at 370 nm. It will not be as powerful as laser excitation but is considerably safer.
Ideal for:
- Optical excitation in visible fluorescence spectroscopy measurements.
- Compatible with modular and optical fiber based systems
Infrared Light Source
This USB-C powered USB Infrared Light Source emits light at specific infrared wavelengths. Infrared radiation is lower intensity than other excitation sources so will minimize damage to more sensitive samples. You can choose from range of wavelengths between 700 - 980 nm. It will not be as powerful as laser excitation but is considerably safer and less expensive.
Ideal for:
- Studying biological samples, functional groups, chemical bonds and molecular Identification
- Compatible with modular and optical fiber based systems
Broadband White Light Source
Our USB Broadband White Light Source outputs light over the entire visible spectrum (360 nm - 900 nm).
Ideal for:
- Absorbance, transmission and reflectivity measurements
- Compatible with modular and optical fiber based systems
LED Light Source
Our range of USB-C powered USB LED Light Source emit light centered around a single wavelength for specific excitation. You can choose from a range of specific wavelengths between 375-980 nm. This can be useful for
Suitable for:
- Fluorescence excitation sources & for optical density measurements
- Compatible with modular and optical fiber based systems
In combination, these two light sources will provide you with everything you need to take basic spectroscopy measurements. These are compatible with standard spectroscopy fittings (SMA 905 fiber connections, M6 optical breadboard holes) so that they can be easily integrated with any spectroscopy system.
LED Light Sources Set
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