{"product_id":"spectrofluorometer","title":"Spectrofluorometer","description":"\u003ch2 id=\"product-oneliner\"\u003eHigh Sensitivity, Bench-Friendly Spectrofluorometer at an Accessible Price\u003c\/h2\u003e\n\u003cp class=\"text-center\"\u003eMeasure absorption, transmission, fluorescence emission, and fluorescence excitation\u003c\/p\u003e\n\u003chr\u003e\n\u003cp class=\"text-center\"\u003e\u003ca href=\"#overview\"\u003eOverview\u003c\/a\u003e | \u003ca href=\"#working-principles\"\u003eHow Spectrofluorometers Work\u003c\/a\u003e | \u003ca href=\"#specifications\"\u003eSpecifications\u003c\/a\u003e | \u003ca href=\"#choosing-a-spectrofluorometer\"\u003eChoosing a Spectrofluorometer\u003c\/a\u003e | \u003ca href=\"#what-spectrofluorometers-measure\"\u003eMeasurements\u003c\/a\u003e\u003cbr\u003e\u003ca href=\"#software\"\u003eSoftware\u003c\/a\u003e | \u003ca href=\"#gallery\"\u003eGallery\u003c\/a\u003e | \u003ca href=\"#components\"\u003eUnderstanding the Components\u003c\/a\u003e | \u003ca href=\"#included\"\u003eIn The Box\u003c\/a\u003e | \u003ca href=\"#resources\"\u003eResources and Support\u003c\/a\u003e\u003c\/p\u003e\n\u003chr\u003e\n\u003cp id=\"overview\"\u003eCombining a dedicated sample holder, light sources, monochromators, and single-pixel detectors in a stand-alone unit ensures consistent alignment between components for reliable measurements. This usually results in spectrofluorometers being quite large and bulky, but with a total footprint of less than 30cm x 30cm, the Ossila Spectrofluorometer is compact enough to fit on any lab bench or worktop.\u003c\/p\u003e\n\u003cp\u003eThe two integrated light sources support different measurements. The hyperspectral white LED is well-suited for absorption, transmission, and fluorescence excitation measurements, while the UV light provides excitation light for fluorescence emission measurements. Both sources have adjustable output power, short warm-up times, and longer lifetimes than traditional fluorescence spectroscopy light sources. On the detection side, the two integrated single-pixel silicon photomultipliers (SiPMs) with active temperature correction are more sensitive than standard photodiodes and offer a wide dynamic range.\u003c\/p\u003e\n\u003cp\u003eThe included sample holder supports both thin films and standard cuvettes, and can be rotated in indexed 22.5-degree increments. The integrated filter holders, meanwhile, allow bandpass filters to be easily inserted into the beam path.\u003c\/p\u003e\n\u003cdiv class=\"row display-flex\"\u003e\n\u003cdiv class=\"col-xs-12 col-sm-4 col-md-3 text-center margin-top\"\u003e\n\u003cimg loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/Icon-Compact.svg?v\" width=\"50\" height=\"50\" alt=\"\"\u003e\n\u003cp class=\"blue-heading no-margin text-center\"\u003eCompact, Integrated System\u003c\/p\u003e\n\u003cp class=\"text-center\"\u003eLess than 30 cm x 30 cm with all components included\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-sm-4 col-md-3 text-center margin-top\"\u003e\n\u003cimg loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/Icon_Simple_to_use_Software.svg?v\" width=\"50\" height=\"50\" alt=\"\"\u003e\n\u003cp class=\"blue-heading no-margin text-center\"\u003eIntuitive Software\u003c\/p\u003e\n\u003cp class=\"text-center\"\u003eSuport for multiple measurement types and periodic measurements\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-sm-4 col-md-3 text-center margin-top\"\u003e\n\u003cimg loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/Icon_Low_Motor_Noise.svg?v\" width=\"50\" height=\"50\" alt=\"\"\u003e\n\u003cp class=\"blue-heading no-margin text-center\"\u003eHighly Sensitive Detectors\u003c\/p\u003e\n\u003cp class=\"text-center\"\u003eSilicon photomultipliers provide hassle-free low-light performance\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-sm-4 col-md-3 text-center margin-top\"\u003e\n\u003cimg loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectral-tunability-icon.svg?v\" width=\"50\" height=\"50\" alt=\"\"\u003e\n\u003cp class=\"blue-heading no-margin text-center\"\u003eWide Measurement Range\u003c\/p\u003e\n\u003cp class=\"text-center\"\u003eTwo integrated light sources allow you measure UV-IR with a single device\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cfigure\u003e\u003cvideo crossorigin=\"\" class=\"widescreen\" height=\"477.172\" width=\"848.328\" poster=\"https:\/\/cdn.ossila.com\/videos\/spectrofluorometer-product-overview.jpg\" preload=\"none\" controlslist=\"nodownload\" controls=\"controls\"\u003e\n    \u003csource src=\"https:\/\/cdn.ossila.com\/videos\/spectrofluorometer-product-overview.mp4\" type=\"video\/mp4\"\u003e\n    \u003ctrack src=\"https:\/\/cdn.ossila.com\/videos\/spectrofluorometer-product-overview.vtt\" srclang=\"en\" label=\"English\" kind=\"subtitles\" default=\"\"\u003e\u003c\/video\u003e\u003c\/figure\u003e\n\u003cdiv class=\"panel panel-default\"\u003e\n\u003cdiv class=\"panel-body\"\u003e\n\u003ch3 id=\"working-principles\"\u003eHow Does a Spectrofluorometer Work?\u003c\/h3\u003e\n\u003chr\u003e\n\u003cp\u003eSpectrofluorometers are essential measurement instruments that can take fluorescence (or photoluminescence), absorbance, and transmission measurements. They can do this with a high level of accuracy and repeatability by selecting wavelengths with nanometer resolution using either a dual-monochromator set-up, or a single monochromator with a dedicated single-wavelength UV light source. The ability to select both the excitation and detection wavelength gives spectrofluorometers their flexibility, allowing them to accurately conduct a range of spectroscopy measurements.\u003c\/p\u003e\n\u003cp\u003eThe Ossila spectrofluorometer works by combining different spectrophotometry components in various orientations to enable different measurements.\u003c\/p\u003e\n\u003cp\u003eFor fluorescence measurements, a single wavelength light source is directed towards the sample to excite any fluorophores. This can use the UV light source or combine the broadband light source and excitation monochromator to create a variable light source. Which source is best depends on the measurement you are taking. Any emitted light from the sample enters the detection monochromator. This monochromator either selects a specific emission wavelength, or scans through various wavelengths before intensity is measured by the detector. \u003c\/p\u003e\n\u003cp\u003eFor absorbance measurements, the broadband light source travels through the excitation monochromator, which again selects a specific wavelength or scans through a wavelength range. This monochromatic light is directed straight through the sample, where transmission is measured by a separate detector\u003c\/p\u003e\n\u003cfigure class=\"double-float-left\"\u003e\u003cimg alt=\"Spectrofluorometer diagram used for fluorescence measurements\" class=\"fill\" height=\"532\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrofluorometer-fluorescence-measurement.jpg?\" width=\"593\"\u003e\n\u003cfigcaption\u003eSetup for fluorescence measurements\u003c\/figcaption\u003e\n\u003c\/figure\u003e\n\u003cfigure class=\"double-float-right\"\u003e\u003cimg alt=\"Spectrofluorometer diagram used for absorbance measurements\" class=\"fill\" height=\"532\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrofluorometer-excitation-spectrum.jpg?\" width=\"593\"\u003e\n\u003cfigcaption\u003eSetup for absorbance measurements\u003c\/figcaption\u003e\n\u003c\/figure\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 id=\"specifications\"\u003eSpecifications\u003c\/h2\u003e\n\u003chr\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth width=\"38.2%\"\u003eSpectral Range (Broadband)\u003c\/th\u003e\n\u003ctd\u003e380 – 1000 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth width=\"38.2%\"\u003eWavelength Accuracy\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;1 nm (in calibrated range)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth width=\"38.2%\"\u003eWavelength Repeatability\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.1 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth width=\"38.2%\"\u003eUV Source Wavelength\u003c\/th\u003e\n\u003ctd\u003e365±15 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth width=\"38.2%\"\u003eSpectral Resolution\u003c\/th\u003e\n\u003ctd\u003eSlit width dependant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth width=\"38.2%\"\u003eSlit Type\u003c\/th\u003e\n\u003ctd\u003eManual – micrometer driven with 10 μm resolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth width=\"38.2%\"\u003eSlit Size\u003c\/th\u003e\n\u003ctd\u003e50 μm – 6 mm (W) x 10 mm (H)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth width=\"38.2%\"\u003eMaximum Sample Size\u003c\/th\u003e\n\u003ctd\u003e75 (W) x 50 (H) x 5 (D) mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth width=\"38.2%\"\u003eCuvette Size\u003c\/th\u003e\n\u003ctd\u003e10 x 10 mm; maximum height 52 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth width=\"38.2%\"\u003eDimensions\u003c\/th\u003e\n\u003ctd\u003e280 x 260 x 100 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth width=\"38.2%\"\u003eWeight\u003c\/th\u003e\n\u003ctd\u003e4.2 kg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cfigure\u003e\u003cimg src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrofluorometer-wireframe.svg?v=1774880091\" alt=\"Ossila Spectrofluorometer dimensions\" height=\"438.75\" loading=\"lazy\" width=\"636\"\u003e\n\u003cfigcaption\u003eOssila Spectrofluorometer wireframe\u003c\/figcaption\u003e\n\u003c\/figure\u003e\n\u003ch2 id=\"choosing-a-spectrofluorometer\"\u003eChoosing a Spectrofluorometer\u003c\/h2\u003e\n\u003chr\u003e\n\u003cp\u003eAs with most measurement equipment, choosing the right spectroscopy system for you comes down to your experimental needs, lab space and budget.\u003c\/p\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cdiv class=\"col-xs-3\"\u003e\n\u003cimg alt=\"Check Mark\" height=\"30\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/Green-Tick.svg\" style=\"float: left; padding-right: 10px;\" width=\"30\"\u003eNon-invasive molecular probing\u003c\/div\u003e\n\u003cdiv class=\"col-xs-3\"\u003e\n\u003cimg alt=\"Check Mark\" height=\"30\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/Green-Tick.svg\" style=\"float: left; padding-right: 10px;\" width=\"30\"\u003eNo complex optical alignment\u003c\/div\u003e\n\u003cdiv class=\"col-xs-3\"\u003e\n\u003cimg alt=\"Check Mark\" height=\"30\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/Green-Tick.svg\" style=\"float: left; padding-right: 10px;\" width=\"30\"\u003eMeasure live dynamic reactions\u003c\/div\u003e\n  \u003cdiv class=\"col-xs-3\"\u003e\n\u003cimg alt=\"Check Mark\" height=\"30\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/Green-Tick.svg\" style=\"float: left; padding-right: 10px;\" width=\"30\"\u003eReliable, calibrated measurements\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"padding-top: 20px\"\u003eThe Ossila Spectrofluorometer is a complete spectrofluorescence system that includes:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eA hyperspectral 380nm – 1000nm white LED \u003c\/li\u003e\n\u003cli\u003eA high-power 365nm UV light source\u003c\/li\u003e\n\u003cli\u003e2 silicon photomultiplier detectors\u003c\/li\u003e\n\u003cli\u003e2 ultra-fast monochromators\u003c\/li\u003e\n\u003cli\u003eA sample chamber with integrated filter holders\u003c\/li\u003e\n\u003cli\u003eSample holders for both solutions and thin films\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cfigure style=\"padding-top: 5px;\"\u003e\u003cimg alt=\"Ossila Spectoflurometer\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrophotometer-in-lab-setup.jpg?crop=center\u0026amp;height=700\u0026amp;width=1340\" width=\"1340\" height=\"700\"\u003e\n\u003cfigcaption\u003eThe Ossila Spectrofluorometer\u003c\/figcaption\u003e\n\u003c\/figure\u003e\n\u003ch3\u003eSpectrofluorometers vs. Spectrophotometers\u003c\/h3\u003e\n\u003cp\u003eSpectrofluorometers are similar to spectrophotometers, and often the terms are used interchangeably. They use the same components, and they build a spectrum in the same way. However, there are a few key differences between spectrofluorometers and spectrophotometers.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth width=\"30%\"\u003e\u003c\/th\u003e\n\u003cth style=\"text-align: center;\"\u003eSpectrofluorometers\u003c\/th\u003e\n\u003cth style=\"text-align: center;\"\u003eSpectrophotometers\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNumber of Monochromators\u003c\/th\u003e\n\u003ctd style=\"text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNumber of Detectors\u003c\/th\u003e\n\u003ctd style=\"text-align: center;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBroadband Light Source\u003c\/th\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cimg alt=\"Includes broadband light source\" height=\"20\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/icon-circle-tick.svg?v=1739952420\" width=\"20\"\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cimg alt=\"Includes broadband light source\" height=\"20\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/icon-circle-tick.svg?v=1739952420\" width=\"20\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUV Light Source\u003c\/th\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cimg alt=\"Includes UV light source\" height=\"20\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/icon-circle-tick.svg?v=1739952420\" width=\"20\"\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cimg alt=\"No UV light source included\" height=\"20\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/icon-blank-circle.svg?v=1739952420\" width=\"20\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSuitable for Absorbance Measurements?\u003c\/th\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cimg alt=\"Suitable for absorbance measurements\" height=\"20\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/icon-circle-tick.svg?v=1739952420\" width=\"20\"\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cimg alt=\"Suitable for absorbance measurements\" height=\"20\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/icon-circle-tick.svg?v=1739952420\" width=\"20\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSuitable for Fluorescence Measurements?\u003c\/th\u003e\n\u003ctd style=\"text-align: center;\"\u003e\n\u003cimg style=\"padding-right: 5px;\" alt=\"Suitable for fluorescence measurements\" height=\"20\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/icon-circle-tick.svg?v=1739952420\" width=\"20\"\u003eEmission and excitation spectra\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cimg alt=\"Not suitable for fluorescence measurements\" height=\"20\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/icon-blank-circle.svg?v=1739952420\" width=\"20\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 id=\"what-spectrofluorometers-measure\"\u003eSpectrofluorometer Measurements\u003c\/h2\u003e\n\u003chr\u003e\n\u003cp\u003eA major benefit of a spectrofluorometer is that you can take different types of measurements with one instrument. The main measurements they are used for are:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFluorescence spectroscopy\u003c\/li\u003e\n\u003cul\u003e\n\u003cli\u003eEmission spectra: Spectrofluorometers can measure the emission spectrum of a fluorophore, where the excitation wavelength is fixed and the emission wavelength is varied. These measurements tell you about the light a fluorophore emits.\u003c\/li\u003e\n\u003cli\u003eExcitation spectra: Where the measured emission wavelength remains the same (ideally where emission is strongest) but the excitation wavelength is varied. These measurements will tell you which wavelengths of light stimulate the most fluorescence.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cli\u003eAbsorbance spectroscopy\u003c\/li\u003e\n\u003cul\u003e\n\u003cli\u003eSpectrofluorometers can also measure absorbance, much like a spectrophotometer. Measuring absorbance with a spectrofluorometer takes longer than with a USB spectrometer but will have higher sensitivity and resolution as you can measure transmission intensity 1 nm at a time.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWhich Setup for Which Measurements?\u003c\/h3\u003e\n\u003cp\u003eTo efficiently use a spectrofluorometer, ensure that you are using the correct combination of spectrofluorometer components.\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth width=\"19%\"\u003e\u003c\/th\u003e\n\u003cth width=\"27%\"\u003eExcitation Fluorescence Measurements\u003c\/th\u003e\n\u003cth width=\"27%\"\u003eEmission Fluorescence Measurements\u003c\/th\u003e\n\u003cth width=\"27%\"\u003eAbsorbance\/Transmission Measurements\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eLight Source Required\u003c\/th\u003e\n\u003ctd\u003eBroadband light source\u003c\/td\u003e\n\u003ctd\u003eSingle wavelength light source\u003c\/td\u003e\n\u003ctd\u003eBroadband light source\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003e\n\u003cp\u003eNumber of Monochromators\u003c\/p\u003e\n\u003c\/th\u003e\n\u003ctd\u003e\n\u003cp\u003eTwo required:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eBetween light source and sample for wavelength scanning\u003c\/li\u003e\n\u003cli\u003eBetween sample and detector for emission wavelength selection\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003ctd\u003eOne required, between the sample and detector\u003c\/td\u003e\n\u003ctd\u003eOne required, between the light source and sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eMore on Fluorescence Spectroscopy\u003c\/h3\u003e\n\u003cp\u003eFluorescence spectroscopy is the study of fluorophores, either for use in fluorescent applications such as in LEDs, or as a marker to study biological processes or chemical reactions. It analyzes light emitted from a sample due to an excited electron relaxing from the first electronic state into the ground state.\u003c\/p\u003e\n\u003cp\u003eThere are two types of fluorescence measurement: steady state fluorescence and time resolved fluorescence. Time resolved PL measurements are used to measure dynamic processes like fluorescence lifetime. Steady state fluorescence can be used to probe the molecular structure of a material and is measured using a spectrofluorometer.\u003c\/p\u003e\n\u003cp\u003eFluorescence spectroscopy is often used alongside absorbance spectroscopy, as absorbance measures the excited state of a molecule whereas fluorescence measurements probe the ground state.\u003c\/p\u003e\n\u003ch2 id=\"software\"\u003eSpectrofluorometer Software\u003c\/h2\u003e\n\u003chr\u003e\n\u003cp\u003eThe Ossila Spectrofluorometer software provides a user-friendly interface for setting measurement parameters and acquiring data, allowing for precise control of fluorescence, absorbance, and transmission measurements.\u003c\/p\u003e\n\u003cfigure\u003e\u003cvideo controls=\"controls\" controlslist=\"nodownload\" preload=\"none\" poster=\"https:\/\/cdn.ossila.com\/videos\/spectrofluorometer-emission-and-excitation.jpg\" width=\"848.328\" height=\"477.172\" class=\"widescreen\" crossorigin=\"\"\u003e\n    \u003csource type=\"video\/mp4\" src=\"https:\/\/cdn.ossila.com\/videos\/spectrofluorometer-emission-and-excitation.mp4\"\u003e\n    \u003ctrack default=\"\" kind=\"subtitles\" label=\"English\" srclang=\"en\" src=\"https:\/\/cdn.ossila.com\/videos\/spectrofluorometer-emission-and-excitation.vtt\"\u003e\u003c\/video\u003e\n\u003cfigcaption\u003eTaking emission and excitation measurements with the Ossila Spectrofluorometer software\u003c\/figcaption\u003e\n\u003c\/figure\u003e\n\u003ch2 id=\"gallery\"\u003eSpectrofluorometer Gallery\u003c\/h2\u003e\n\u003chr\u003e\n\u003cdiv class=\"carousel-container carousel-gallery\"\u003e\n\u003cdiv class=\"carousel-track ease-in-out-transition\"\u003e\n\u003ca href=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrofluorometer-fluorescence.jpg?width=1620\" class=\"carousel-item\" title=\"Fluorescence measurement\"\u003e \u003cimg width=\"420\" height=\"280\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrofluorometer-fluorescence.jpg?width=420\u0026amp;height=280\u0026amp;crop=center\" alt=\"Fluorescence measurement\"\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrofluorometer-450nm-filter-holder.jpg?width=1620\" class=\"carousel-item\" title=\"Spectrofluorometer filter holder with 450 nm  filter\"\u003e \u003cimg width=\"420\" height=\"280\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrofluorometer-450nm-filter-holder.jpg?width=420\u0026amp;height=280\u0026amp;crop=center\" alt=\"Spectrofluorometer filter holder with 450 nm bandpass filter\"\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrofluorometer-filter-holder.jpg?width=1620\" class=\"carousel-item\" title=\"Spectrofluorometer filter holder\"\u003e \u003cimg width=\"420\" height=\"280\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrofluorometer-filter-holder.jpg?width=420\u0026amp;height=280\u0026amp;crop=center\" alt=\"Spectrofluorometer filter holder\"\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrofluorometer-full-setup.jpg?width=1620\" class=\"carousel-item\" title=\"Full spectrofluorometer setup\"\u003e \u003cimg width=\"420\" height=\"280\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrofluorometer-full-setup.jpg?width=420\u0026amp;height=280\u0026amp;crop=center\" alt=\"Full spectrofluorometer setup\"\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrofluorometer-full-setup-and-substrates.jpg?width=1620\" class=\"carousel-item\" title=\"Full spectrofluorometer setup with PC software\"\u003e \u003cimg width=\"420\" height=\"280\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrofluorometer-full-setup-and-substrates.jpg?width=420\u0026amp;height=280\u0026amp;crop=center\" alt=\"Full spectrofluorometer setup with PC software\"\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrofluorometer-setup.jpg?width=1620\" class=\"carousel-item\" title=\"Setting up the spectrofluorometer\"\u003e \u003cimg width=\"420\" height=\"280\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrofluorometer-setup.jpg?width=420\u0026amp;height=280\u0026amp;crop=center\" alt=\"Setting up the spectrofluorometer\"\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrofluorometer-compact-size.jpg?width=1620\" class=\"carousel-item\" title=\"Compact spectrofluorometer\"\u003e \u003cimg width=\"420\" height=\"280\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/spectrofluorometer-compact-size.jpg?width=420\u0026amp;height=280\u0026amp;crop=center\" alt=\"Compact spectrofluorometer\"\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003ca role=\"button\" class=\"carousel-left-control\"\u003e \u003csvg aria-hidden=\"true\" class=\"icon icon-chevron-left\"\u003e\u003cuse href=\"#icon-chevron-left\"\u003e\u003c\/use\u003e\u003c\/svg\u003e \u003cspan class=\"sr-only\"\u003ePrevious \u003c\/span\u003e\u003c\/a\u003e \u003ca role=\"button\" class=\"carousel-right-control\"\u003e \u003csvg aria-hidden=\"true\" class=\"icon icon-chevron-right\"\u003e\u003cuse href=\"#icon-chevron-right\"\u003e\u003c\/use\u003e\u003c\/svg\u003e \u003cspan class=\"sr-only\"\u003eNext \u003c\/span\u003e\u003c\/a\u003e\n\u003c\/div\u003e\n\u003ch2 id=\"components\"\u003eUnderstanding Spectrofluorometer Components\u003c\/h2\u003e\n\u003chr\u003e\n\u003ch3\u003eExcitation Sources\u003c\/h3\u003e\n\u003cp\u003eSingle wavelength light sources, such as the UV light source, provide a high energy excitation source with a narrow wavelength range to selectively excite fluorophores for fluorescence measurements. However, absorbance measurements and excitation fluorescence measurements require a broadband light source so spectrofluorometers also need a broadband light source.\u003c\/p\u003e\n\u003cp\u003eIn most spectrofluorometers, the broadband light source is passed through a scanning monochromator, where wavelengths are selected individually and an absorbance spectrum is built wavelength by wavelength.\u003c\/p\u003e\n\u003cp\u003eThis variable light source can also be used as a narrow wavelength excitation source for fluorescence measurements. Therefore, spectrofluorometers, like the Ossila Spectrofluorometer, often have both a high energy UV light source and a variable light source, allowing users to conduct a wider range of measurements with just one instrument.\u003c\/p\u003e\n\u003ch3\u003eSample Holder\u003c\/h3\u003e\n\u003cp\u003eThe sample holder is a straight-forward but vital part of the system. It ensures consistency between measurements and holds the sample steady. There are sample holders designed to measure substrates for thin film measurement. Additionally, many samples measure fluorescence within solution, usually in a quartz cuvette. Using ultra-flat quartz cuvettes or substrates helps to reduce reflection, scattering and absorbance of light. This ensures that the maximum possible light is transmitted through the holder without interaction.\u003c\/p\u003e\n\u003ch3\u003eDetectors\u003c\/h3\u003e\n\u003cp\u003eSpectrofluorometers use single pixel detectors, unlike spectrometers which use array detectors. Single pixel detectors can only measure intensity and have no way to decipher between different wavelengths of light. This makes spectrofluorometers more sensitive to smaller signals, but means that wavelength selection must take place before or after interacting with the sample. Often photomultipliers are used as these detectors, with the type determining the spectral range and sensitivity of the spectrofluorometer.\u003c\/p\u003e\n\u003cp\u003eFor fluorescence measurements, the light needs to pass through a monochromator before it hits the detector. This isolates the emission wavelength and crucially reducing the intensity of the excitation wavelength in the transmitted signal.\u003c\/p\u003e\n\u003cp\u003eFor absorbance measurements, the light source must be able to pass through the sample directly into the detector. Therefore, for maximum versatility, some spectrofluorometers, such as the Ossila Spectrofluorometer, come with multiple detectors in different locations to facilitate both absorbance and fluorescence measurements.\u003c\/p\u003e\n\u003ch2 id=\"included\"\u003eIn the Box\u003c\/h2\u003e\n\u003chr\u003e\n\u003cul\u003e\n\u003cli\u003eThe Ossila Spectrofluorometer\u003c\/li\u003e\n\u003cli\u003eCuvette holder\u003c\/li\u003e\n\u003cli\u003eThin film holder\u003c\/li\u003e\n\u003cli\u003eUSB-C data cable\u003c\/li\u003e\n\u003cli\u003eUSB drive with software \u0026amp; user manual\u003c\/li\u003e\n\u003cli\u003e24V power supply\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 id=\"resources\"\u003eResources and Support\u003c\/h2\u003e\n\u003chr\u003e\n\u003cdiv class=\"tile-container\"\u003e\n\u003cdiv class=\"card-box card-truncate\"\u003e\n\u003ca href=\"\/pages\/fluorescence-spectroscopy\"\u003e \u003cimg alt=\"Fluorescence Spectroscopy\" loading=\"lazy\" src=\"https:\/\/www.ossila.com\/cdn\/shop\/files\/Fluorescence-Phosphorescence.svg?v=1677850351\"\u003e \u003c\/a\u003e \u003ca href=\"\/pages\/fluorescence-spectroscopy\"\u003eFluorescence Spectroscopy\u003c\/a\u003e\n\u003cp\u003eFluorescence spectroscopy is used to measure fluorescence. The technique often used together with absorbance spectroscopy. Fluorescence is a type of photoluminescence where light is quickly reemitted from a material after incident photons are absorbed. This is different to phosphorescence where there is a delay between photon absorption and emission. The term fluorescence is often used interchangeably with photoluminescence.\u003c\/p\u003e\n\u003ca href=\"\/pages\/fluorescence-spectroscopy\"\u003eRead more...\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"card-box card-truncate\"\u003e\n\u003ca href=\"https:\/\/www.ossila.com\/pages\/spectrophotometry\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0823\/0287\/files\/monochromator-mirror-reflection-550nm-light.jpg?width=160\u0026amp;height=160\u0026amp;crop=center\" loading=\"lazy\" height=\"160\" alt=\"What Is Spectrophotometry?\"\u003e\u003c\/a\u003e \u003ca href=\"https:\/\/www.ossila.com\/pages\/spectrophotometry\"\u003eWhat Is Spectrophotometry?\u003c\/a\u003e\n\u003cp\u003eSpectrophotometry examines the interactions between visible light and matter through measurements like absorbance, transmission, and reflectance spectroscopy.\u003c\/p\u003e\n\u003ca href=\"https:\/\/www.ossila.com\/pages\/spectrophotometry\"\u003eRead more...\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Ossila","offers":[{"title":"Default Title","offer_id":51505889542360,"sku":"G3000A1","price":16500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0823\/0287\/files\/spectrofluorometer-product-photo.jpg?v=1774951309","url":"https:\/\/www.ossila.com\/products\/spectrofluorometer","provider":"Ossila","version":"1.0","type":"link"}